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Persistent Cyfip1 Expression Is Required to Maintain the Adult Subventricular Zone Neurogenic Niche.
Habela, Christa Whelan; Yoon, Ki-Jun; Kim, Nam-Shik; Taga, Arens; Bell, Kassidy; Bergles, Dwight E; Maragakis, Nicholas J; Ming, Guo-Li; Song, Hongjun.
Afiliación
  • Habela CW; Department of Neurology, shongjun@pennmedicine.upenn.edu chabela1@jhmi.edu.
  • Yoon KJ; Department of Neurology.
  • Kim NS; Korea Advanced Institute of Science and Technology, College of Life Science and Bioengineering, Department of Biological Sciences, Daejeon 34141, Republic of Korea.
  • Taga A; Department of Neuroscience and Mahoney Institute for Neurosciences.
  • Bell K; Department of Neurology.
  • Bergles DE; Department of Neuroscience and Mahoney Institute for Neurosciences.
  • Maragakis NJ; Department of Neurology.
  • Ming GL; Department of Neurology.
  • Song H; Solomon H. Snyder Department of Neuroscience, Johns Hopkins University Baltimore, Maryland 21287.
J Neurosci ; 40(10): 2015-2024, 2020 03 04.
Article en En | MEDLINE | ID: mdl-31988061
ABSTRACT
Neural stem cells (NSCs) persist throughout life in the subventricular zone (SVZ) neurogenic niche of the lateral ventricles as Type B1 cells in adult mice. Maintaining this population of NSCs depends on the balance between quiescence and self-renewing or self-depleting cell divisions. Interactions between B1 cells and the surrounding niche are important in regulating this balance, but the mechanisms governing these processes have not been fully elucidated. The cytoplasmic FMRP-interacting protein (Cyfip1) regulates apical-basal polarity in the embryonic brain. Loss of Cyfip1 during embryonic development in mice disrupts the embryonic niche and affects cortical neurogenesis. However, a direct role for Cyfip1 in the regulation of adult NSCs has not been established. Here, we demonstrate that Cyfip1 expression is preferentially localized to B1 cells in the adult mouse SVZ. Loss of Cyfip1 in the embryonic mouse brain results in altered adult SVZ architecture and expansion of the adult B1 cell population at the ventricular surface. Furthermore, acute deletion of Cyfip1 in adult NSCs results in a rapid change in adherens junction proteins as well as increased proliferation and number of B1 cells at the ventricular surface. Together, these data indicate that Cyfip1 plays a critical role in the formation and maintenance of the adult SVZ niche; furthermore, deletion of Cyfip1 unleashes the capacity of adult B1 cells for symmetric renewal to increase the adult NSC pool.SIGNIFICANCE STATEMENT Neural stem cells (NSCs) persist in the subventricular zone of the lateral ventricles in adult mammals, and the size of this population is determined by the balance between quiescence and self-depleting or renewing cell division. The mechanisms regulating these processes are not fully understood. This study establishes that the cytoplasmic FMRP interacting protein 1 (Cyfip1) regulates NSC fate decisions in the adult subventricular zone and adult NSCs that are quiescent or typically undergo self-depleting divisions retain the ability to self-renew. These results contribute to our understanding of how adult NSCs are regulated throughout life and has potential implications for human brain disorders.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Ventrículos Laterales / Proteínas Adaptadoras Transductoras de Señales / Nicho de Células Madre / Neurogénesis / Células-Madre Neurales Límite: Animals Idioma: En Revista: J Neurosci Año: 2020 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Ventrículos Laterales / Proteínas Adaptadoras Transductoras de Señales / Nicho de Células Madre / Neurogénesis / Células-Madre Neurales Límite: Animals Idioma: En Revista: J Neurosci Año: 2020 Tipo del documento: Article