Characterization of the structure and interactions of P450 BM3 using hybrid mass spectrometry approaches.

Jeffreys, Laura N; Pacholarz, Kamila J; Johannissen, Linus O; Girvan, Hazel M; Barran, Perdita E; Voice, Michael W; Munro, Andrew W

Jeffreys, Laura N; Pacholarz, Kamila J; Johannissen, Linus O; Girvan, Hazel M; Barran, Perdita E; Voice, Michael W; Munro, Andrew W.

Afiliación

Jeffreys LN; The Manchester Institute of Biotechnology, School of Natural Sciences, Department of Chemistry, The University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom.
Pacholarz KJ; Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), The University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom.
Johannissen LO; Michael Barber Centre for Collaborative Mass Spectrometry, Manchester Institute of Biotechnology, The University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom.
Girvan HM; The Manchester Institute of Biotechnology, School of Natural Sciences, Department of Chemistry, The University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom.
Barran PE; Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), The University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom.
Voice MW; The Manchester Institute of Biotechnology, School of Natural Sciences, Department of Chemistry, The University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom.
Munro AW; Manchester Synthetic Biology Research Centre for Fine and Speciality Chemicals (SYNBIOCHEM), The University of Manchester, 131 Princess Street, Manchester M1 7DN, United Kingdom.

J Biol Chem ; 295(22): 7595-7607, 2020 05 29.

Article en En | MEDLINE | ID: mdl-32303637

Asunto(s)

Bacillus megaterium/enzimología; Proteínas Bacterianas/química; Sistema Enzimático del Citocromo P-450/química; NADPH-Ferrihemoproteína Reductasa/química; Multimerización de Proteína; Cristalografía por Rayos X; Medición de Intercambio de Deuterio; Espectrometría de Masas; Dominios Proteicos; Estructura Cuaternaria de Proteína

Palabras clave

collision induced unfolding (CIU); cytochrome P450; cytochrome P450 BM3; dimerization; domain interactions; enzyme catalysis; enzyme structure; flavoprotein; fusion protein; hydrogen-deuterium exchange mass spectrometry (HDX-MS); mass spectrometry (MS); monooxygenase; native ion mobility mass spectrometry (IM-MS); protein dynamics; protein solvent accessibility; reductase; solution structure; structural biology

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Bacillus megaterium / Proteínas Bacterianas / NADPH-Ferrihemoproteína Reductasa / Sistema Enzimático del Citocromo P-450 / Multimerización de Proteína Tipo de estudio: Prognostic_studies Idioma: En Revista: J Biol Chem Año: 2020 Tipo del documento: Article País de afiliación: Reino Unido

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