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Acquisition of Plasmid-Mediated Colistin Resistance Gene mcr-1 in Escherichia coli of Livestock Origin in Bangladesh.
Dutta, Avijit; Islam, Md Zohorul; Barua, Himel; Rana, Eaftekhar Ahmed; Jalal, Mohammad Shah; Dhar, Pangkaj Kumar; Das, Avijit; Das, Tridip; Sarma, Sabuj Manohar; Biswas, Sanjoy Kanti; Biswas, Paritosh Kumar.
Afiliación
  • Dutta A; Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University (CVASU), Chattogram, Bangladesh.
  • Islam MZ; Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University (CVASU), Chattogram, Bangladesh.
  • Barua H; Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University (CVASU), Chattogram, Bangladesh.
  • Rana EA; Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University (CVASU), Chattogram, Bangladesh.
  • Jalal MS; Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University (CVASU), Chattogram, Bangladesh.
  • Dhar PK; Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University (CVASU), Chattogram, Bangladesh.
  • Das A; Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University (CVASU), Chattogram, Bangladesh.
  • Das T; Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University (CVASU), Chattogram, Bangladesh.
  • Sarma SM; Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University (CVASU), Chattogram, Bangladesh.
  • Biswas SK; Department of Microbiology, Chattogram-Maa-O-Shishu Hospital Medical College, Chattogram, Bangladesh.
  • Biswas PK; Department of Microbiology and Veterinary Public Health, Chattogram Veterinary and Animal Sciences University (CVASU), Chattogram, Bangladesh.
Microb Drug Resist ; 26(9): 1058-1062, 2020 Sep.
Article en En | MEDLINE | ID: mdl-32349645
Aims: To investigate plasmid-borne colistin resistance mechanism (plasmid-mediated colistin resistance [mcr-1]) in Escherichia coli of human, veterinary, and environmental origin in Bangladesh. Materials and methods: A total of 810 samples were collected from different sources. Isolation and identification of E. coli was performed using classical bacteriology and then tested for antimicrobial susceptibility. Colistin-resistant isolates were further analyzed for mcr-1 gene using PCR. Minimum inhibitory concentration (MIC) was determined using microbroth dilution technique. After sequencing of mcr-1 gene, phylogenetics was conducted to see the relationship with other mcr-1 gene sequences. Results: A total of 358 E. coli were isolated from 810 samples of humans, animals, environment, and food in Bangladesh. Of them 49 (15.9%) isolates were phenotypically resistant to colistin and 254 (70.9%) were resistant to multiple antimicrobials. mcr-1 gene was detected in three E. coli isolates of poultry source. For the three mcr-1 positive isolates the MIC of colistin sulfate was 4, 8, and 128 µg/mL. Gene sequencing of two of the three mcr-1 positive isolates and phylogenetic analysis showed close similarities of one isolate to other mcr-1 sequences available at GenBank while the other appeared to have evolved locally. Conclusion: First-ever report on circulation of mcr-1 E. coli of livestock origin in Bangladesh.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas de Escherichia coli / Farmacorresistencia Bacteriana / Escherichia coli / Infecciones por Escherichia coli Tipo de estudio: Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Animals / Humans País/Región como asunto: Asia Idioma: En Revista: Microb Drug Resist Asunto de la revista: MICROBIOLOGIA / TERAPIA POR MEDICAMENTOS Año: 2020 Tipo del documento: Article País de afiliación: Bangladesh

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas de Escherichia coli / Farmacorresistencia Bacteriana / Escherichia coli / Infecciones por Escherichia coli Tipo de estudio: Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Animals / Humans País/Región como asunto: Asia Idioma: En Revista: Microb Drug Resist Asunto de la revista: MICROBIOLOGIA / TERAPIA POR MEDICAMENTOS Año: 2020 Tipo del documento: Article País de afiliación: Bangladesh