Cloning, overexpression, purification, characterization and structural modelling of a metabolically active Fe<sup>2+</sup> dependent 2,6-dichloro-p-hydroquinone 1,2-dioxygenase (CpsA) from Bacillus cereus strain AOA-CPS_1.

Aregbesola, Oladipupo A; Kumar, Ajit; Mokoena, Mduduzi P; Olaniran, Ademola O

Cloning, overexpression, purification, characterization and structural modelling of a metabolically active Fe²⁺ dependent 2,6-dichloro-p-hydroquinone 1,2-dioxygenase (CpsA) from Bacillus cereus strain AOA-CPS_1.

Aregbesola, Oladipupo A; Kumar, Ajit; Mokoena, Mduduzi P; Olaniran, Ademola O.

Afiliación

Aregbesola OA; Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban 4000, Republic of South Africa.
Kumar A; Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban 4000, Republic of South Africa.
Mokoena MP; Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban 4000, Republic of South Africa.
Olaniran AO; Discipline of Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal (Westville Campus), Private Bag X54001, Durban 4000, Republic of South Africa. Electronic address: olanirana@ukzn.ac.za.

Int J Biol Macromol ; 161: 247-257, 2020 Oct 15.

Article en En | MEDLINE | ID: mdl-32512093

Asunto(s)

Bacillus cereus/genética; Bacillus cereus/metabolismo; Clonación Molecular; Dioxigenasas/genética; Dioxigenasas/metabolismo; Expresión Génica; Hierro/metabolismo; Secuencia de Aminoácidos; Activación Enzimática; Cromatografía de Gases y Espectrometría de Masas; Concentración de Iones de Hidrógeno; Modelos Moleculares; Filogenia; Conformación Proteica; Proteínas Recombinantes; Relación Estructura-Actividad; Temperatura

Palabras clave

2,6-Dichloro-p-hydroquinone; 2,6-Dichloro-p-hydroquinone 1,2-dioxygenase; Bacillus cereus; Pentachlorophenol

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Bacillus cereus / Expresión Génica / Clonación Molecular / Dioxigenasas / Hierro Tipo de estudio: Prognostic_studies Idioma: En Revista: Int J Biol Macromol Año: 2020 Tipo del documento: Article

Texto completo

Imprimir

XML

PubMed Links

Buscar en Google