Heat Shock Protein 90 Involvement in the Development of Idiopathic Epiretinal Membranes.
Invest Ophthalmol Vis Sci
; 61(8): 34, 2020 07 01.
Article
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| MEDLINE
| ID: mdl-32716502
ABSTRACT
Purpose:
This work was aimed to further characterize cells of idiopathic epiretinal membranes (iERMs). We wanted to determine the contribution of 90-kDa heat shock protein (HSP90) to sustain the transforming growth factor-ß (TGF-ß)-mediated signal transduction pathway in iERM.Methods:
Immunofluorescence and confocal microscopy were carried out on deplasticized sections from 36 epiretinal membranes processed for electron microscopy and on frozen sections from five additional samples with antibodies against α-smooth muscle actin (αSMA), vimentin, glial fibrillary acidic protein (GFAP), SMAD2, HSP90α, type-II TGF-ß1 receptor (TßRII), type-I collagen, and type-IV collagen. In addition, Müller MIO-M1 cells were transfected with HSP90 and challenged with TGF-ß1.Results:
Double and triple labeling experiments showed that a variable number of TßRII+ cells were present in 94.1% of tested iERMs and they were mostly GFAP-/αSMA+/vimentin+/HSP90α+. In almost half of the cases these cells contained type-I collagen, suggesting their involvement in matrix deposition. HSP90 overexpressing MIO-M1 cells challenged with TGF-ß1 showed increased levels of TßRII, SMAD2, SMAD3, and phosphor-SMAD2. Nuclear SMAD2 staining could be observed in HSP90α+ cells on frozen sections of iERMs.Conclusions:
Cells in iERMs that express TßRII are also HSP90α+ and show the antigenic profile of myofibroblast-like cells as they are GFAP-/αSMA+/vimentin+. HSP90α-overexpressing MIO-M1 cells challenged with TGF-ß1 showed an increased activation of the SMAD pathway implying that HSP90α might play a role in sustaining the TGF-ß1-induced fibrotic response of iERM cells.
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Banco de datos:
MEDLINE
Asunto principal:
Factor de Crecimiento Transformador beta
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Proteínas HSP90 de Choque Térmico
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Membrana Epirretinal
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Proteínas Smad
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Factor de Crecimiento Transformador beta1
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Células Ependimogliales
Límite:
Humans
Idioma:
En
Revista:
Invest Ophthalmol Vis Sci
Año:
2020
Tipo del documento:
Article