lncRNA FEZF1AS1 promotes migration, invasion and epithelialmesenchymal transition of retinoblastoma cells by targeting miR12363p.
Mol Med Rep
; 22(5): 3635-3644, 2020 Nov.
Article
en En
| MEDLINE
| ID: mdl-32901841
ABSTRACT
Long noncoding RNAs (lncRNAs) and microRNAs (miRs) have been reported to regulate disease progression in numerous types of disease, including retinoblastoma (Rb). Therefore, the present study aimed to investigate the effects of the lncRNA FEZ family zinc finger 1 antisense RNA 1 (FEZF1AS1) on Rb and to determine its possible mechanism of action. Reverse transcriptionquantitative PCR and western blot analysis were conducted to detect the gene or protein expression. Cell Counting Kit8, wound healing and transwell invasion assays were performed to estimate the capabilities of cell viability, invasion and migration. The potential association between FEZF1AS1 and miR12363p in Y79 cells was measured via dualluciferase reporter assay. The results of the present study revealed that the levels of FEZF1AS1 were significantly upregulated in different Rb cell lines, with the most prominent upregulation observed in Y79 cells. In addition, the cell viability, invasive and migratory abilities, and the ability to undergo epithelialmesenchymal transition (EMT), were significantly inhibited following the transfection of short hairpin RNA (shRNA)FEZF1AS1 into Y79 cells. Further experimental validation confirmed that miR12363p may be a direct target of FEZF1AS1. Notably, the miR12363p inhibitor was discovered to reverse the inhibitory effects of shRNAFEZF1AS1 on cell viability, invasion, migration and EMT. In conclusion, the findings of the present study suggested that lncRNAFEZF1AS1 may promote the viability, migration, invasion and EMT of Rb cells by modulating miR12363p.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Retinoblastoma
/
Regulación hacia Arriba
/
MicroARNs
/
ARN Largo no Codificante
Límite:
Humans
Idioma:
En
Revista:
Mol Med Rep
Año:
2020
Tipo del documento:
Article