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Segmented flow generator for serial crystallography at the European X-ray free electron laser.
Echelmeier, Austin; Cruz Villarreal, Jorvani; Messerschmidt, Marc; Kim, Daihyun; Coe, Jesse D; Thifault, Darren; Botha, Sabine; Egatz-Gomez, Ana; Gandhi, Sahir; Brehm, Gerrit; Conrad, Chelsie E; Hansen, Debra T; Madsen, Caleb; Bajt, Sasa; Meza-Aguilar, J Domingo; Oberthür, Dominik; Wiedorn, Max O; Fleckenstein, Holger; Mendez, Derek; Knoska, Juraj; Martin-Garcia, Jose M; Hu, Hao; Lisova, Stella; Allahgholi, Aschkan; Gevorkov, Yaroslav; Ayyer, Kartik; Aplin, Steve; Ginn, Helen Mary; Graafsma, Heinz; Morgan, Andrew J; Greiffenberg, Dominic; Klujev, Alexander; Laurus, Torsten; Poehlsen, Jennifer; Trunk, Ulrich; Mezza, Davide; Schmidt, Bernd; Kuhn, Manuela; Fromme, Raimund; Sztuk-Dambietz, Jolanta; Raab, Natascha; Hauf, Steffen; Silenzi, Alessandro; Michelat, Thomas; Xu, Chen; Danilevski, Cyril; Parenti, Andrea; Mekinda, Leonce; Weinhausen, Britta; Mills, Grant.
Afiliación
  • Echelmeier A; School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287-1604, USA.
  • Cruz Villarreal J; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Messerschmidt M; School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287-1604, USA.
  • Kim D; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Coe JD; School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287-1604, USA.
  • Thifault D; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Botha S; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Egatz-Gomez A; School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287-1604, USA.
  • Gandhi S; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Brehm G; School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287-1604, USA.
  • Conrad CE; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Hansen DT; School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287-1604, USA.
  • Madsen C; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Bajt S; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Meza-Aguilar JD; Department of Physics, Arizona State University, Tempe, AZ, 85287-1504, USA.
  • Oberthür D; School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287-1604, USA.
  • Wiedorn MO; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Fleckenstein H; School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287-1604, USA.
  • Mendez D; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Knoska J; Institute for X-Ray Physics, University of Göttingen, Friedrich-Hund-Platz 1, 37077, Göttingen, Germany.
  • Martin-Garcia JM; School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287-1604, USA.
  • Hu H; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Lisova S; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Allahgholi A; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Gevorkov Y; Department of Physics, Arizona State University, Tempe, AZ, 85287-1504, USA.
  • Ayyer K; Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
  • Aplin S; Centre for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.
  • Ginn HM; European XFEL, Holzkoppel 4, 22869, Schenefeld, Germany.
  • Graafsma H; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
  • Morgan AJ; Centre for Ultrafast Imaging, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.
  • Greiffenberg D; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
  • Klujev A; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
  • Laurus T; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Poehlsen J; Department of Physics, Arizona State University, Tempe, AZ, 85287-1504, USA.
  • Trunk U; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
  • Mezza D; Department of Physics, Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.
  • Schmidt B; School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287-1604, USA.
  • Kuhn M; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Fromme R; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Sztuk-Dambietz J; Department of Physics, Arizona State University, Tempe, AZ, 85287-1504, USA.
  • Raab N; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, 85287-7401, USA.
  • Hauf S; Department of Physics, Arizona State University, Tempe, AZ, 85287-1504, USA.
  • Silenzi A; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
  • Michelat T; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
  • Xu C; Hamburg University of Technology, Vision Systems E-2, Harburger Schloßstraße 20, 21079, Hamburg, Germany.
  • Danilevski C; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
  • Parenti A; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
  • Mekinda L; Division of Structural Biology, University of Oxford, Oxford, OX1 2JD, United Kingdom.
  • Weinhausen B; Diamond Light Source Ltd, Didcot, Oxfordshire, OX11 0DE, United Kingdom.
  • Mills G; Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron, Notkestrasse 85, 22607, Hamburg, Germany.
Nat Commun ; 11(1): 4511, 2020 09 09.
Article en En | MEDLINE | ID: mdl-32908128
ABSTRACT
Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-resolved crystallography. Common liquid sample delivery continuously jets the protein crystal suspension into the path of the XFEL, wasting a vast amount of sample due to the pulsed nature of all current XFEL sources. The European XFEL (EuXFEL) delivers femtosecond (fs) X-ray pulses in trains spaced 100 ms apart whereas pulses within trains are currently separated by 889 ns. Therefore, continuous sample delivery via fast jets wastes >99% of sample. Here, we introduce a microfluidic device delivering crystal laden droplets segmented with an immiscible oil reducing sample waste and demonstrate droplet injection at the EuXFEL compatible with high pressure liquid delivery of an SFX experiment. While achieving ~60% reduction in sample waste, we determine the structure of the enzyme 3-deoxy-D-manno-octulosonate-8-phosphate synthase from microcrystals delivered in droplets revealing distinct structural features not previously reported.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Cristalografía / Electrones / Dispositivos Laboratorio en un Chip / Rayos Láser Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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XML
PubMed Links
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Cristalografía / Electrones / Dispositivos Laboratorio en un Chip / Rayos Láser Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos