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Genome-Wide Differential DNA Methylation and miRNA Expression Profiling Reveals Epigenetic Regulatory Mechanisms Underlying Nitrogen-Limitation-Triggered Adaptation and Use Efficiency Enhancement in Allotetraploid Rapeseed.
Hua, Ying-Peng; Zhou, Ting; Huang, Jin-Yong; Yue, Cai-Peng; Song, Hai-Xing; Guan, Chun-Yun; Zhang, Zhen-Hua.
Afiliación
  • Hua YP; School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
  • Zhou T; School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
  • Huang JY; School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
  • Yue CP; School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China.
  • Song HX; Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, College of Resources and Environmental Sciences, Hunan Agricultural University, Changsha 430128, China.
  • Guan CY; National Center of Oilseed Crop Improvement, Hunan Branch, Changsha 430128, China.
  • Zhang ZH; Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, College of Resources and Environmental Sciences, Hunan Agricultural University, Changsha 430128, China.
Int J Mol Sci ; 21(22)2020 Nov 10.
Article en En | MEDLINE | ID: mdl-33182819
ABSTRACT
Improving crop nitrogen (N) limitation adaptation (NLA) is a core approach to enhance N use efficiency (NUE) and reduce N fertilizer application. Rapeseed has a high demand for N nutrients for optimal plant growth and seed production, but it exhibits low NUE. Epigenetic modification, such as DNA methylation and modification from small RNAs, is key to plant adaptive responses to various stresses. However, epigenetic regulatory mechanisms underlying NLA and NUE remain elusive in allotetraploid B. napus. In this study, we identified overaccumulated carbohydrate, and improved primary and lateral roots in rapeseed plants under N limitation, which resulted in decreased plant nitrate concentrations, enhanced root-to-shoot N translocation, and increased NUE. Transcriptomics and RT-qPCR assays revealed that N limitation induced the expression of NRT1.1, NRT1.5, NRT1.7, NRT2.1/NAR2.1, and Gln1;1, and repressed the transcriptional levels of CLCa, NRT1.8, and NIA1. High-resolution whole genome bisulfite sequencing characterized 5094 differentially methylated genes involving ubiquitin-mediated proteolysis, N recycling, and phytohormone metabolism under N limitation. Hypermethylation/hypomethylation in promoter regions or gene bodies of some key N-metabolism genes might be involved in their transcriptional regulation by N limitation. Genome-wide miRNA sequencing identified 224 N limitation-responsive differentially expressed miRNAs regulating leaf development, amino acid metabolism, and plant hormone signal transduction. Furthermore, degradome sequencing and RT-qPCR assays revealed the miR827-NLA pathway regulating limited N-induced leaf senescence as well as the miR171-SCL6 and miR160-ARF17 pathways regulating root growth under N deficiency. Our study provides a comprehensive insight into the epigenetic regulatory mechanisms underlying rapeseed NLA, and it will be helpful for genetic engineering of NUE in crop species through epigenetic modification of some N metabolism-associated genes.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Brassica napus / Epigénesis Genética / Nitrógeno Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Brassica napus / Epigénesis Genética / Nitrógeno Idioma: En Revista: Int J Mol Sci Año: 2020 Tipo del documento: Article País de afiliación: China