The attachment process and physiological properties of Escherichia coli O157:H7 on quartz.
BMC Microbiol
; 20(1): 355, 2020 11 19.
Article
en En
| MEDLINE
| ID: mdl-33213384
ABSTRACT
BACKGROUND:
Manure application and sewage irrigation release many intestinal pathogens into the soil. After being introduced into the soil matrix, pathogens are commonly found to attach to soil minerals. Although the survival of mineral-associated Escherichia coli O157H7 has been studied, a comprehensive understanding of the attachment process and physiological properties after attachment is still lacking.RESULTS:
In this study, planktonic and attached Escherichia coli O157H7 cells on quartz were investigated using RNA sequencing (RNA-seq) and the isobaric tagging for relative and absolute quantitation (iTRAQ) proteomic method. Based on the transcriptomic and proteomic analyses and gene knockouts, functional two-component system pathways were required for efficient attachment; chemotaxis and the Rcs system were identified to play determinant roles in E. coli O157H7 attachment on quartz. After attachment, the pyruvate catabolic pathway shifted from the tricarboxylic acid (TCA) cycle toward the fermentative route. The survival rate of attached E. coli O157H7 increased more than 10-fold under penicillin and vancomycin stress and doubled under alkaline pH and ferric iron stress.CONCLUSIONS:
These results contribute to the understanding of the roles of chemotaxis and the Rcs system in the attachment process of pathogens and indicate that the attachment of pathogens to minerals significantly elevates their resistance to antibiotics and environmental stress, which may pose a potential threat to public health.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Cuarzo
/
Adhesión Bacteriana
/
Escherichia coli O157
Idioma:
En
Revista:
BMC Microbiol
Asunto de la revista:
MICROBIOLOGIA
Año:
2020
Tipo del documento:
Article
País de afiliación:
China