Protocol for Tri-culture of hiPSC-Derived Neurons, Astrocytes, and Microglia.
STAR Protoc
; 1(3): 100190, 2020 12 18.
Article
en En
| MEDLINE
| ID: mdl-33377084
ABSTRACT
This protocol establishes a tri-culture of hiPSC-derived neurons, astrocytes, and microglia for the study of cellular interactions during homeostasis, injury, and disease. This system allows for mechanistic studies that can identify the roles of individual cell types in disease and injury response in a physiologically relevant, all-human system. This protocol utilizes and modifies prior differentiations. Limitations include the prolonged maturation of human astrocytes and neurons and scalability. For complete details on the use and execution of this protocol, please refer to Ryan et al. (2020).
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Cocultivo
/
Técnicas de Cultivo de Célula
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos