Isolation of human ESC-derived cardiac derivatives and embryonic heart cells for population and single-cell RNA-seq analysis.
STAR Protoc
; 2(1): 100339, 2021 03 19.
Article
en En
| MEDLINE
| ID: mdl-33644774
ABSTRACT
The combination of population and single-cell RNA sequencing analysis using human embryonic stem cell (hESC) differentiation and developmental tissues is a powerful approach to elucidate an organ-specific cellular and molecular atlas in human embryogenesis. This protocol describes (1) cardiac-directed differentiation and isolation of hESC-derived cardiac derivatives with fluorescence-activated cell sorting, (2) isolation of human embryonic heart-derived single cardiac cells, and (3) construction of cDNA libraries with Smart-seq2. These allow for the preparation of human developmental samples for comprehensive transcriptional analysis. For complete details on the use and execution of this protocol, please refer to Sahara et al. (2019).
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Diferenciación Celular
/
Análisis de la Célula Individual
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Citometría de Flujo
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Células Madre Embrionarias Humanas
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RNA-Seq
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Miocardio
Límite:
Humans
Idioma:
En
Revista:
STAR Protoc
Año:
2021
Tipo del documento:
Article
País de afiliación:
Suecia