Detection of Cu2+ and S2- with fluorescent polymer nanoparticles and bioimaging in HeLa cells.
Anal Bioanal Chem
; 413(15): 3945-3953, 2021 Jun.
Article
en En
| MEDLINE
| ID: mdl-33954830
ABSTRACT
Novel spherical polymer nanoparticles were synthesized by hyperbranched polyethylenimine (hPEI) and 6-hydroxy-2-naphthaldehyde (HNA) via Schiff base reaction (one-pot reaction), which had great advantages in water solubility and green synthesis. Meanwhile, probe PEI-HNA could quickly detect Cu2+ in the range of 0-60 µM in 30 s with the detection limit of 243 nM. The fluorescence of PEI-HNA-Cu2+ could be recovered by the addition of S2- in 50 s with the detection limit of 227 nM. Based on the excellent optical properties, PEI-HNA has been used in the bioimaging of living cells with excellent cell penetrability and low toxicity. More importantly, PEI-HNA has been doped into filter paper, hydrogel, and nanofibrous film to prepare solid-phase sensors, displaying rapid response and excellent sensitivity. Moreover, the low-cost and simple preparation of these sensors offers great potential and possibilities for industrialization, which could help accelerate the development of sensors in environmental and biological fields.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Polímeros
/
Azufre
/
Cobre
/
Nanopartículas
/
Colorantes Fluorescentes
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2021
Tipo del documento:
Article
País de afiliación:
China