Immunofluorescence microscopy-based assessment of cytosolic DNA accumulation in mammalian cells.
STAR Protoc
; 2(2): 100488, 2021 06 18.
Article
en En
| MEDLINE
| ID: mdl-34041502
ABSTRACT
Here, we describe an immunofluorescence (IF) microscopy-based approach to quantify cytosolic double-stranded DNA molecules in cultured eukaryotic cells upon the selective and specific permeabilization of plasma membranes. This technique is compatible with widefield microscopy coupled with automated image analysis for mid- to high-throughput applications and high-resolution confocal microscopy for subcellular assessments and co-localization studies. In addition to enabling single-cell and subcellular resolution, this approach circumvents most constraints associated with alternative approaches based on subcellular fractionation. For complete use and execution of this protocol, please refer to Yamazaki et al. (2020).
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
ADN
/
Citosol
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Análisis de la Célula Individual
/
Microscopía Fluorescente
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2021
Tipo del documento:
Article
País de afiliación:
Estados Unidos