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Dynamic crotonylation of EB1 by TIP60 ensures accurate spindle positioning in mitosis.
Song, Xiaoyu; Yang, Fengrui; Liu, Xu; Xia, Peng; Yin, Wu; Wang, Zhikai; Wang, Yong; Yuan, Xiao; Dou, Zhen; Jiang, Kai; Ma, Mingming; Hu, Bing; Zhang, Rui; Xu, Chao; Zhang, Zhiyong; Ruan, Ke; Tian, Ruijun; Li, Lin; Liu, Tao; Hill, Donald L; Zang, Jianye; Liu, Xing; Li, Jinsong; Cheng, Jinke; Yao, Xuebiao.
Afiliación
  • Song X; MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
  • Yang F; Keck Center for Organoids Plasticity, Morehouse School of Medicine, Atlanta, GA, USA.
  • Liu X; MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
  • Xia P; Keck Center for Organoids Plasticity, Morehouse School of Medicine, Atlanta, GA, USA.
  • Yin W; MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
  • Wang Z; Keck Center for Organoids Plasticity, Morehouse School of Medicine, Atlanta, GA, USA.
  • Wang Y; MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
  • Yuan X; MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
  • Dou Z; MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
  • Jiang K; Keck Center for Organoids Plasticity, Morehouse School of Medicine, Atlanta, GA, USA.
  • Ma M; Frontier Science Center for Immunology and Metabolism, Medical Research Institute, Wuhan University, Wuhan, China.
  • Hu B; MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
  • Zhang R; Department of Chemistry, Southern University of Science and Technology, Shenzhen, China.
  • Xu C; MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
  • Zhang Z; Frontier Science Center for Immunology and Metabolism, Medical Research Institute, Wuhan University, Wuhan, China.
  • Ruan K; MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
  • Tian R; MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
  • Li L; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, USA.
  • Liu T; MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
  • Hill DL; MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
  • Zang J; MOE Key Laboratory for Cellular Dynamics and Hefei National Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
  • Liu X; Department of Chemistry, Southern University of Science and Technology, Shenzhen, China.
  • Li J; Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, China.
  • Cheng J; State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing, China.
  • Yao X; Comprehensive Cancer Center, University of Alabama, Birmingham, AL, USA.
Nat Chem Biol ; 17(12): 1314-1323, 2021 12.
Article en En | MEDLINE | ID: mdl-34608293
ABSTRACT
Spindle position control is essential for cell fate determination and organogenesis. Early studies indicate the essential role of the evolutionarily conserved Gαi/LGN/NuMA network in spindle positioning. However, the regulatory mechanisms that couple astral microtubules dynamics to the spindle orientation remain elusive. Here we delineated a new mitosis-specific crotonylation-regulated astral microtubule-EB1-NuMA interaction in mitosis. EB1 is a substrate of TIP60, and TIP60-dependent crotonylation of EB1 tunes accurate spindle positioning in mitosis. Mechanistically, TIP60 crotonylation of EB1 at Lys66 forms a dynamic link between accurate attachment of astral microtubules to the lateral cell cortex defined by NuMA-LGN and fine tune of spindle positioning. Real-time imaging of chromosome movements in HeLa cells expressing genetically encoded crotonylated EB1 revealed the importance of crotonylation dynamics for accurate control of spindle orientation during metaphase-anaphase transition. These findings delineate a general signaling cascade that integrates protein crotonylation with accurate spindle positioning for chromosome stability in mitosis.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas de Ciclo Celular / Lisina Acetiltransferasa 5 / Proteínas Asociadas a Microtúbulos / Microtúbulos / Huso Acromático Límite: Humans Idioma: En Revista: Nat Chem Biol Asunto de la revista: BIOLOGIA / QUIMICA Año: 2021 Tipo del documento: Article País de afiliación: China
Texto completo
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas de Ciclo Celular / Lisina Acetiltransferasa 5 / Proteínas Asociadas a Microtúbulos / Microtúbulos / Huso Acromático Límite: Humans Idioma: En Revista: Nat Chem Biol Asunto de la revista: BIOLOGIA / QUIMICA Año: 2021 Tipo del documento: Article País de afiliación: China