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A label-free electrochemical immunosensor for CA125 detection based on CMK-3(Au/Fc@MgAl-LDH)n multilayer nanocomposites modification.
Wu, Mengdie; Liu, Simin; Qi, Feifan; Qiu, Ren; Feng, Jing; Ren, Xinshui; Rong, Shengzhong; Ma, Hongkun; Chang, Dong; Pan, Hongzhi.
Afiliación
  • Wu M; Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China; Shanghai University of Medicine and Health Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai, 200093, China.
  • Liu S; Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China; School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
  • Qi F; Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China; School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
  • Qiu R; Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China; School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
  • Feng J; The College of Medical Technology, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China.
  • Ren X; Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China; Shanghai University of Medicine and Health Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai, 200093, China.
  • Rong S; Public Health School, Mudanjiang Medical University, Mudanjiang, 157011, China.
  • Ma H; Public Health School, Mudanjiang Medical University, Mudanjiang, 157011, China.
  • Chang D; Department of Clinical Laboratory, The Affiliated Pudong Hospital, Fudan University, Shanghai, 201399, China. Electronic address: dongchang1969@163.com.
  • Pan H; Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China. Electronic address: panhongzhilaoshi@163.com.
Talanta ; 241: 123254, 2022 May 01.
Article en En | MEDLINE | ID: mdl-35101834
A label-free electrochemical immunosensor was constructed for cancer antigen 125 (CA125) detection based on multiple-enlargement means of layer-by-layer (LBL) assembly of ordered mesoporous carbon (CMK-3), gold nanoparticles (Au NPs) and MgAl layered double hydroxides containing ferrocenecarboxylic acid (Fc@MgAl-LDH). A CMK-3(Au/Fc@MgAl-LDH)n multilaminate nanocomposites was designed using technology of LBL self-assembly among negatively charged Au NPs, positively charged CMK-3 and Fc@MgAl-LDH nanosheets. The CMK-3(Au/Fc@MgAl-LDH)n multilaminate nanocomposites was used as carriers to increase the immobilization of antibody and the number of loading Fc, conductors to strengthen conductivity and enhancers to amplify signal of Fc step-by-step. Besides, this special and excellent way of LBL assembly can immensely amplify the signal of immunosensor and more immobilize the biomolecules, and label-free method is a more simple the measuring way and the procedure. The immunosensor displayed a wider linear range of 0.01 U ml-1-1000 U ml-1 and a lower detection limit of 0.004 U ml-1. Therefore, the sensor can stablely and accurately be applied for CA125 detection in clinical cancer diagnosis.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Nanocompuestos / Nanopartículas del Metal / Grafito Tipo de estudio: Diagnostic_studies Idioma: En Revista: Talanta Año: 2022 Tipo del documento: Article País de afiliación: China
Texto completo
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Técnicas Biosensibles / Nanocompuestos / Nanopartículas del Metal / Grafito Tipo de estudio: Diagnostic_studies Idioma: En Revista: Talanta Año: 2022 Tipo del documento: Article País de afiliación: China