LC-MS/MS methods to quantify HCP002 in human plasma and urine: applications in a pharmacokinetic study.
Bioanalysis
; 14(5): 307-316, 2022 Mar.
Article
en En
| MEDLINE
| ID: mdl-35195039
ABSTRACT
Aim:
HCP002, a phosphate-modified derivative of voriconazole, can improve solubility without using the nephrotoxic solubilizer, sulfobutylether-ß-cyclodextrin. To study pharmacokinetics in humans, LC-MS/MS methods to quantify HCP002 in human plasma and urine were developed and validated.Method:
After protein precipitation by acetonitrile containing voriconazole-d3, HCP002 was separated on a ZORBAX SB-Aq column, and LCMS/MS analysis was performed in multi-response monitoring mode.Results:
The analytical run time was 3 min. Linearity was observed over the ranges of 0.100-40.0 and 0.400-200 µg/ml in plasma and urine, respectively. Precision and accuracy were within acceptable limits. Sample stability was confirmed.Conclusion:
Rapid and reproducible methods quantified HCP002 in urine, and plasma samples were established.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Plasma
/
Espectrometría de Masas en Tándem
Límite:
Humans
Idioma:
En
Revista:
Bioanalysis
Año:
2022
Tipo del documento:
Article
País de afiliación:
China