Recombinant cysteine proteinase as anti-tick targeting Hyalomma asiaticum infestation.
Exp Parasitol
; 235: 108234, 2022 Apr.
Article
en En
| MEDLINE
| ID: mdl-35218759
ABSTRACT
Cysteine proteases are involved in the digestion of host blood and the degradation of yolk proteins of arthropod ectoparasites. In this study, a cathepsin L-like cysteine proteinase gene (HasCPL) of Hyalomma asiaticum was cloned, and recombinant (r)HasCPL protein was generated for immunization study. Bioinformatic analysis confirmed HasCPL was a member of the papain family (clan CA) and have high sequence identities with CPLs of other Ixodid ticks. The efficacy of immunization against H. asiaticum infestations in rabbits was assessed. Rabbits (n = 3) were immunized three times with rHasCPL before challenged with 250 larvae per rabbit four weeks post-immunization. A high antibody titer was detected in immunized rabbits in comparison to control. Western blot analysis detected CPLs in midgut, salivary gland, and ovary. Increase of rejection percentage of larvae were noted in ticks fed on immunized animals in comparison to control. Overall, a 55.09% protection against larva ticks was noted.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Infestaciones por Garrapatas
/
Ixodidae
/
Proteasas de Cisteína
Límite:
Animals
Idioma:
En
Revista:
Exp Parasitol
Año:
2022
Tipo del documento:
Article