The Main Structural Unit Elucidation and Immunomodulatory Activity In Vitro of a Selenium-Enriched Polysaccharide Produced by Pleurotus ostreatus.
Molecules
; 27(8)2022 Apr 18.
Article
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| MEDLINE
| ID: mdl-35458788
ABSTRACT
In recent years, the structure of selenium-enriched polysaccharides and their application in immunomodulation have attracted much attention. In previous studies, we extracted and purified a novel selenium-enriched Pleurotus ostreatus polysaccharide called Se-POP-21, but its structure and immunomodulatory activity were still unclear. In this study, the main structural unit formula of Se-POP-21 was characterized by methylation analysis and an NMR experiment. The results showed that the backbone of Se-POP-21 was â[2,6)-α-D-Galp-(1â6)-α-D-Galp-(1]4â2,4)-ß-L-Arap-(1â[2,6)-α-D-Galp-(1â6)-α-D-Galp-(1]4â, branched chain of ß-D-Manp-(1â and ß-D-Manp-(1â4)-ß-L-Arap-(1â connected with â2,6)-α-D-Galp-(1â and â2,4)-ß-L-Arap-(1â,respectively, through the O-2 bond. In vitro cell experiments indicated that Se-POP-21 could significantly enhance the proliferation and phagocytosis of RAW264.7 cells, upregulate the expression of costimulatory molecules CD80/CD86, and promote RAW264.7 cells to secrete NO, ROS, TNF-α, IL-1ß, and IL-6 by activating the NF-κB protein. The results of this study indicate that Se-POP-21 can effectively activate RAW264.7 cells. Thus, it has the potential to be used in immunomodulatory drugs or functional foods.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Selenio
/
Pleurotus
Límite:
Animals
Idioma:
En
Revista:
Molecules
Asunto de la revista:
BIOLOGIA
Año:
2022
Tipo del documento:
Article
País de afiliación:
China