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Technical pipeline for screening microbial communities as a function of substrate specificity through fluorescent labelling.
Leivers, Shaun; Lagos, Leidy; Garbers, Philipp; La Rosa, Sabina Leanti; Westereng, Bjørge.
Afiliación
  • Leivers S; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ch.M.Falsens vei 1, 1432, Aas, Norway. shaun.allan.leivers@nmbu.no.
  • Lagos L; Faculty of Biosciences, Norwegian University of Life Sciences, Oluf Thesens vei 6, 1433, Aas, Norway.
  • Garbers P; Skretting ARC, Sjøhagen 3, 4016, Stavanger, Norway.
  • La Rosa SL; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ch.M.Falsens vei 1, 1432, Aas, Norway.
  • Westereng B; Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ch.M.Falsens vei 1, 1432, Aas, Norway.
Commun Biol ; 5(1): 444, 2022 05 11.
Article en En | MEDLINE | ID: mdl-35545700
The study of specific glycan uptake and metabolism is an effective tool in aiding with the continued unravelling of the complexities in the human gut microbiome. To this aim fluorescent labelling of glycans may provide a powerful route towards this target. Here, we successfully used the fluorescent label 2-aminobenzamide (2-AB) to monitor and study microbial degradation of labelled glycans. Both single strain and co-cultured fermentations of microbes from the common human-gut derived Bacteroides genus, are able to grow when supplemented with 2-AB labelled glycans of different monosaccharide composition, degrees of acetylation and polymerization. Utilizing a multifaceted approach that combines chromatography, mass spectrometry, microscopy and flow cytometry techniques, it is possible to better understand the metabolism of labelled glycans in both supernatants and at a single cell level. We envisage this combination of complementary techniques will help further the understanding of substrate specificity and the role it plays within microbial communities.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Microbiota / Microbioma Gastrointestinal Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Humans Idioma: En Revista: Commun Biol Año: 2022 Tipo del documento: Article País de afiliación: Noruega

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Microbiota / Microbioma Gastrointestinal Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Humans Idioma: En Revista: Commun Biol Año: 2022 Tipo del documento: Article País de afiliación: Noruega