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Establishment and characterization of IPS-OGC-C1: a novel induced pluripotent stem cell line from healthy human ovarian granulosa cells.
Wang, Zhiqiang; Hu, Xiaojia; He, Qiufen; Lai, Jingbo; Pan, Ruolang; Zheng, Jing; Chen, Ye.
Afiliación
  • Wang Z; Department of Genetics, Department of Genetic and Metabolic Disease, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310052, Zhejiang, China.
  • Hu X; Zhejiang Provincial Key Laboratory of Genetic and Developmental Disorders, Institute of Genetics, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
  • He Q; Department of Genetics, Department of Genetic and Metabolic Disease, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310052, Zhejiang, China.
  • Lai J; Zhejiang Provincial Key Laboratory of Genetic and Developmental Disorders, Institute of Genetics, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
  • Pan R; Zhejiang Provincial Key Laboratory of Genetic and Developmental Disorders, Institute of Genetics, Zhejiang University, Hangzhou, 310058, Zhejiang, China.
  • Zheng J; Reproductive Medical Center, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310004, Zhejiang, China.
  • Chen Y; Department of Genetics, Department of Genetic and Metabolic Disease, The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou, 310052, Zhejiang, China.
Hum Cell ; 35(5): 1612-1620, 2022 Sep.
Article en En | MEDLINE | ID: mdl-35876985
Ovarian granulosa cell (OGC) is a critical somatic component of the ovary, which provides physical support and the microenvironment required for the developing oocyte. Human OGCs are easy to obtain and culture as a by-product of follicular aspiration performed during in vitro fertilization (IVF) procedures. Therefore, OGCs offer a potent cell source to generate induced pluripotent stem cells (iPSCs). This study established a novel OGCs-derived iPSC cell line from the follicular fluid of a healthy female donor with a Chinese Han genetic background and named it IPS-OGC-C1. IPS-OGC-C1 was verified for embryonic stem cell morphology, cell marker expression, alkaline phosphatase (AP) activity, transcriptomic profile, and pluripotency capability in developing all three embryonic germ layers in vivo and in vitro.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Madre Pluripotentes Inducidas Límite: Female / Humans Idioma: En Revista: Hum Cell Año: 2022 Tipo del documento: Article País de afiliación: China
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Madre Pluripotentes Inducidas Límite: Female / Humans Idioma: En Revista: Hum Cell Año: 2022 Tipo del documento: Article País de afiliación: China