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Nonhematogenic circulating aneuploid cells confer inferior prognosis and therapeutic resistance in gliomas.
Li, Mingxiao; Gao, Faliang; Ren, Xiaohui; Dong, Gehong; Chen, Hongyan; Lin, Alexander Y; Wang, Daisy Dandan; Liu, Mingyang; Lin, Peter Ping; Shen, Shaoping; Jiang, Haihui; Yang, Chuanwei; Zhang, Xiaokang; Zhao, Xuzhe; Zhu, Qinghui; Li, Ming; Cui, Yong; Lin, Song.
Afiliación
  • Li M; Department of Neuro-Surgical Oncology, National Clinical Research Center for Neurological Diseases, Capital Medical University, Beijing, China.
  • Gao F; Department of Neurosurgery, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.
  • Ren X; Department of Neurosurgery, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang, China.
  • Dong G; Key Laboratory of Endocrine Gland Diseases of Zhejiang Province, Hangzhou, Zhejiang, China.
  • Chen H; Department of Neuro-Surgical Oncology, National Clinical Research Center for Neurological Diseases, Capital Medical University, Beijing, China.
  • Lin AY; Department of Neurosurgery, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.
  • Wang DD; Department of Pathology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
  • Liu M; Department of Radiology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.
  • Lin PP; Cytelligen, San Diego, California, USA.
  • Shen S; Cytelligen, San Diego, California, USA.
  • Jiang H; Department of Medicine, University of Oklahoma Health Science Center, Oklahoma City, Oklahoma, USA.
  • Yang C; Cytelligen, San Diego, California, USA.
  • Zhang X; Department of Neuro-Surgical Oncology, National Clinical Research Center for Neurological Diseases, Capital Medical University, Beijing, China.
  • Zhao X; Department of Neurosurgery, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.
  • Zhu Q; Department of Neuro-Surgical Oncology, National Clinical Research Center for Neurological Diseases, Capital Medical University, Beijing, China.
  • Li M; Department of Neurosurgery, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.
  • Cui Y; Department of Neuro-Surgical Oncology, National Clinical Research Center for Neurological Diseases, Capital Medical University, Beijing, China.
  • Lin S; Department of Neurosurgery, Beijing Neurosurgical Institute, Capital Medical University, Beijing, China.
Cancer Sci ; 113(10): 3535-3546, 2022 Oct.
Article en En | MEDLINE | ID: mdl-35940591
ABSTRACT
Aneuploidy is the hallmark of malignancy. Our previous study successfully detected nonhematogenic circulating aneuploidy cells (CACs) in types of gliomas. The current prospective clinical study aims to further precisely subcategorize aneuploid CACs, including CD31- circulating tumor cells (CTCs) and CD31+ circulating tumor endothelial cells, and thoroughly investigate the clinical utilities of these different subtypes of cells. Co-detection and analysis of CTCs and circulating tumor-derived endothelial cells (CTECs) expressing CD133, glial fibrillary acidic protein (GFAP), or epidermal growth factor receptor variant III (EGFR vIII) were performed by integrated subtraction enrichment and immunostaining fluorescence in situ hybridization (SE-iFISH) in 111 preoperative primary diffuse glioma patients. Aneuploid CACs could be detected in most de novo glioma patients. Among detected CACs, 45.6% were CD31- /CD45- aneuploid CTCs and the remaining 54.4% were CD31+ /CD45- aneuploid CTECs. Positive detection of CTECs significantly correlated with disruption of the blood-brain barrier. The median number of large CTCs (L CTCs, >5 µm, 2) in low-grade glioma (WHO grade 2) was less than high-grade glioma (WHO grades 3 and 4) (3, p = 0.044), but this difference was not observed in small CTCs (S CTCs, ≤5 µm), CTECs or CACs (CTCs + CTECs). The numbers of CTCs, CTECs, or CACs in patients with contrast-enhancing (CE) lesions considerably exceeded that of non-CE lesions (p < 0.05). Receiver operating characteristic curves demonstrated that CD31+ CTECs, especially L CTECs, exhibited a close positive relationship with CE lesions. Survival analysis revealed that the high number of CD31- CTCs could be an adverse factor for compromised progression-free survival and overall survival. Longitudinal surveillance of CD31- CTCs was suitable for evaluating the therapeutic response and for monitoring potential emerging treatment resistance.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Glioma / Células Neoplásicas Circulantes Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Cancer Sci Año: 2022 Tipo del documento: Article País de afiliación: China
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Glioma / Células Neoplásicas Circulantes Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Cancer Sci Año: 2022 Tipo del documento: Article País de afiliación: China