Immunohistochemical comparison of lateral bone augmentation using a synthetic TiO2 block or a xenogeneic graft in chronic alveolar defects.
Clin Implant Dent Relat Res
; 25(1): 57-67, 2023 Feb.
Article
en En
| MEDLINE
| ID: mdl-36222116
ABSTRACT
OBJECTIVES:
To evaluate osteogenic markers and alveolar ridge profile changes in guided bone regeneration (GBR) of chronic noncontained bone defects using a nonresorbable TiO2 block. MATERIALS ANDMETHODS:
Three buccal bone defects were created in each hemimandible of eight beagle dogs and allowed to heal for 8 weeks before GBR. Treatment was assigned by block randomization TiO2 block TiO2 -scaffold and a collagen membrane, DBBM particulates Deproteinized bovine bone mineral (DBBM) and a collagen membrane, Empty control Only collagen membrane. Bone regeneration was assessed on two different healing timepoints early (4 weeks) and late healing (12 weeks) using several immunohistochemistry markers including alpha-smooth muscle actin (α-SMA), osteopontin, osteocalcin, tartrate-resistant acid phosphatase, and collagen type I. Histomorphometry was performed on Movat Pentachrome-stained and Von Kossa/Van Gieson-stained sections. Stereolithographic (STL) models were used to compare alveolar profile changes.RESULTS:
The percentage of α-SMA and osteopontin increased in TiO2 group after 12 weeks of healing at the bone-scaffold interface, while collagen type I increased in the empty control group. In the defect area, α-SMA decreased in the empty control group, while collagen type I increased in the DBBM group. All groups maintained alveolar profile from 4 to 12 weeks, but TiO2 group demonstrated the widest soft tissue contour profile.CONCLUSIONS:
The present findings suggested contact osteogenesis when GBR is performed with a TiO2 block or DBBM particulates. The increase in osteopontin indicated a potential for bone formation beyond 12 weeks. The alveolar profile data indicated a sustained lateral increase in lateral bone augmentation using a TiO2 block and a collagen membrane, as compared with DBBM and a collagen membrane or a collagen membrane alone.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Sustitutos de Huesos
/
Aumento de la Cresta Alveolar
Tipo de estudio:
Clinical_trials
/
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Clin Implant Dent Relat Res
Asunto de la revista:
ODONTOLOGIA
Año:
2023
Tipo del documento:
Article
País de afiliación:
Noruega