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An improved imaging system that corrects MS2-induced RNA destabilization.
Li, Weihan; Maekiniemi, Anna; Sato, Hanae; Osman, Christof; Singer, Robert H.
Afiliación
  • Li W; Program in RNA Biology, Albert Einstein College of Medicine, The Bronx, NY, USA. weihan.li@einsteinmed.edu.
  • Maekiniemi A; Department of Cell Biology, Albert Einstein College of Medicine, The Bronx, NY, USA. weihan.li@einsteinmed.edu.
  • Sato H; Program in RNA Biology, Albert Einstein College of Medicine, The Bronx, NY, USA. anna.maekiniemi@einsteinmed.edu.
  • Osman C; Department of Cell Biology, Albert Einstein College of Medicine, The Bronx, NY, USA. anna.maekiniemi@einsteinmed.edu.
  • Singer RH; Program in RNA Biology, Albert Einstein College of Medicine, The Bronx, NY, USA.
Nat Methods ; 19(12): 1558-1562, 2022 12.
Article en En | MEDLINE | ID: mdl-36357695
ABSTRACT
The MS2 and MS2-coat protein (MS2-MCP) imaging system is widely used to study messenger RNA (mRNA) spatial distribution in living cells. Here, we report that the MS2-MCP system destabilizes some tagged mRNAs by activating the nonsense-mediated mRNA decay pathway. We introduce an improved version, which counteracts this effect by increasing the efficiency of translation termination of the tagged mRNAs. Improved versions were developed for both yeast and mammalian systems.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Proteínas de la Cápside Límite: Animals Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Proteínas de la Cápside Límite: Animals Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos