Your browser doesn't support javascript.
loading
Decorin Promotes Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells.
Adachi, Orie; Sugii, Hideki; Itoyama, Tomohiro; Fujino, Shoko; Kaneko, Hiroshi; Tomokiyo, Atsushi; Hamano, Sayuri; Hasegawa, Daigaku; Obata, Junko; Yoshida, Shinichiro; Kadowaki, Masataka; Sugiura, Risa; Albougha, Mhd Safwan; Maeda, Hidefumi.
Afiliación
  • Adachi O; Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
  • Sugii H; Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
  • Itoyama T; Department of Endodontology, Kyushu University Hospital, Fukuoka 812-8582, Japan.
  • Fujino S; Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
  • Kaneko H; Department of Endodontology, Kyushu University Hospital, Fukuoka 812-8582, Japan.
  • Tomokiyo A; Department of Endodontology, Kyushu University Hospital, Fukuoka 812-8582, Japan.
  • Hamano S; Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
  • Hasegawa D; OBT Research Center, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
  • Obata J; Department of Endodontology, Kyushu University Hospital, Fukuoka 812-8582, Japan.
  • Yoshida S; Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
  • Kadowaki M; Department of Endodontology, Kyushu University Hospital, Fukuoka 812-8582, Japan.
  • Sugiura R; Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
  • Albougha MS; Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
  • Maeda H; Department of Endodontology and Operative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
Molecules ; 27(23)2022 Nov 25.
Article en En | MEDLINE | ID: mdl-36500314
ABSTRACT
The aim of this study is to clarify the biological functions of decorin (DCN) in the healing and regeneration of wounded periodontal tissue. We investigated the expression pattern of DCN during the healing of wounded periodontal tissue in rats by immunohistochemistry and the effects of DCN on the osteoblastic differentiation of human periodontal ligament (PDL) stem cells (HPDLSCs) and preosteoblasts by Alizarin red S staining, quantitative reverse transcription-polymerase chain reactions, and western blotting. The expression of DCN was increased around the wounded PDL tissue on day 5 after surgery compared with the nonwounded PDL tissue, whereas its expression was not changed in the osteoblastic layer around the wounded alveolar bone. Furthermore, DCN promoted the osteoblastic differentiation of HPDLSCs, but it did not affect the osteoblastic differentiation of preosteoblasts. ERK1/2 phosphorylation was upregulated during the DCN-induced osteoblastic differentiation of HPDLSCs. DCN did not affect proliferation, migration, or the PDL-related gene expression of HPDLSCs. In conclusion, this study demonstrates that DCN has a role in the healing of wounded periodontal tissue. Furthermore, DCN secreted from PDL cells may contribute to bone healing by upregulating osteoblastic differentiation through ERK1/2 signaling in HPDLSCs, indicating a therapeutic effect of DCN in periodontal tissue regeneration.
Asunto(s)
Palabras clave

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Ligamento Periodontal / Células Madre Límite: Animals / Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Ligamento Periodontal / Células Madre Límite: Animals / Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2022 Tipo del documento: Article País de afiliación: Japón