An interactive web application for processing, correcting, and visualizing genome-wide pooled CRISPR-Cas9 screens.
Cell Rep Methods
; 3(1): 100373, 2023 01 23.
Article
en En
| MEDLINE
| ID: mdl-36814834
ABSTRACT
A limitation of pooled CRISPR-Cas9 screens is the high false-positive rate in detecting essential genes arising from copy-number-amplified genomics regions. To solve this issue, we previously developed CRISPRcleanR a computational method implemented as R/python package and in a dockerized version. CRISPRcleanR detects and corrects biased responses to CRISPR-Cas9 targeting in an unsupervised fashion, accurately reducing false-positive signals while maintaining sensitivity in identifying relevant genetic dependencies. Here, we present CRISPRcleanR WebApp , a web application enabling access to CRISPRcleanR through an intuitive interface. CRISPRcleanR WebApp removes the complexity of R/python language user interactions; provides user-friendly access to a complete analytical pipeline, not requiring any data pre-processing and generating gene-level summaries of essentiality with associated statistical scores; and offers a range of interactively explorable plots while supporting a more comprehensive range of CRISPR guide RNAs' libraries than the original package. CRISPRcleanR WebApp is available at https//crisprcleanr-webapp.fht.org/.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Genoma
/
Sistemas CRISPR-Cas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Cell Rep Methods
Año:
2023
Tipo del documento:
Article
País de afiliación:
Italia