Computational approach to evaluate scRNA-seq data quality and gene body coverage with SkewC.
STAR Protoc
; 4(1): 102038, 2023 03 17.
Article
en En
| MEDLINE
| ID: mdl-36853658
ABSTRACT
SkewC is a single-cell RNA sequencing (scRNA-seq) data quality evaluation tool. The approach is based on determining gene body coverage, and its skewness, as a quality metric for each individual cell. SkewC distinguishes between two types of single cells typical cells with prototypical gene body coverage profiles and skewed cells with skewed gene body coverage profiles. SkewC can be used on any scRNA-seq data as it is independent from the underlying technology used to generate the data. For complete details on the use and execution of this protocol, please refer to Abugessaisa et al. (2022).1.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Perfilación de la Expresión Génica
/
Exactitud de los Datos
Idioma:
En
Revista:
STAR Protoc
Año:
2023
Tipo del documento:
Article
País de afiliación:
Japón