Principles and applications of green fluorescent protein-based biosensors: a mini-review.
Analyst
; 148(13): 2882-2891, 2023 Jun 26.
Article
en En
| MEDLINE
| ID: mdl-37255369
ABSTRACT
Green fluorescent proteins (GFPs) are useful and essential biomolecules that have revolutionized biosensor research. Researchers have extensively utilized GFPs for designing fluorescence biosensors due to their intrinsic fluorescence, high stability, and ability to undergo permutation or mutation. This review provides a concise summary of recent advancements in developing GFP-based biosensors. The construction approaches for GFP-based biosensors can be categorized into four types (1) single GFP-based biosensors; (2) fluorescence resonance energy transfer-based biosensors; (3) GFP-based split biosensors; and (4) GFP chromophore analogy-based biosensors. We highlight the applications of these sensors in biomolecule detection and life sciences, while also sharing personal insights into the challenges associated with GFP-based biosensors and proposing future research directions.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
Idioma:
En
Revista:
Analyst
Año:
2023
Tipo del documento:
Article
País de afiliación:
China