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Fluorometric Determination of DNA Nanostructure Biostability.
Talbot, Hannah; Madhanagopal, Bharath Raj; Hayden, Andrew; Halvorsen, Ken; Chandrasekaran, Arun Richard.
Afiliación
  • Talbot H; The RNA Institute, University at Albany, State University of New York, Albany, New York 12203, United States.
  • Madhanagopal BR; The RNA Institute, University at Albany, State University of New York, Albany, New York 12203, United States.
  • Hayden A; The RNA Institute, University at Albany, State University of New York, Albany, New York 12203, United States.
  • Halvorsen K; The RNA Institute, University at Albany, State University of New York, Albany, New York 12203, United States.
  • Chandrasekaran AR; The RNA Institute, University at Albany, State University of New York, Albany, New York 12203, United States.
ACS Appl Bio Mater ; 6(8): 3074-3078, 2023 08 21.
Article en En | MEDLINE | ID: mdl-37262927
The analysis and improvement of DNA nanostructure biostability is one of the keys areas of progress needed in DNA nanotechnology applications. Here, we present a plate-compatible fluorometric assay for measuring DNA nanostructure biostability using the common intercalator ethidium bromide. We demonstrate the assay by testing the biostability of duplex DNA, a double crossover DNA motif, and a DNA origami nanostructure against different nucleases and in fetal bovine serum. This method scales well to measure a large number of samples using a plate reader and can complement existing methods for assessing and developing robust DNA nanostructures.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Nanoestructuras Idioma: En Revista: ACS Appl Bio Mater Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
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PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Nanoestructuras Idioma: En Revista: ACS Appl Bio Mater Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos