Inhibition of MLCKmediated migration and invasion in human endometriosis stromal cells by NFκB inhibitor DHMEQ.
Mol Med Rep
; 28(2)2023 Aug.
Article
en En
| MEDLINE
| ID: mdl-37326118
ABSTRACT
Endometriosis is initiated by the movement of endometrial cells in the uterus to the fallopian tubes, the ovaries and the peritoneal cavity after the shedding of the uterus lining. To cause endometriosis, it is often necessary for these endometrial cells to migrate, invade and grow at the secondary site. In the present study, immortalized human endometriosis stromal cells (HESC) were employed to look for the inhibitors of migration and invasion. Using a chemical library of bioactive metabolites, it was found that an NFκB inhibitor, DHMEQ, inhibited the migration and invasion of HESC. Both wholegenome array and metastasis PCR array analyses suggested the involvement of myosin light chain kinase (MLCK) in the mechanism of inhibition. DHMEQ was confirmed to inhibit the expression of MLCK and small inhibitory RNA knockdown of MLCK reduced cellular migration and invasion. The addition of DHMEQ to the knockdown cells did not further inhibit migration and invasion. DHMEQ is particularly effective in suppressing disease models by intraperitoneal (IP) administration and this therapy is being developed for the treatment of inflammation and cancer. DHMEQ IP therapy may also be useful for the treatment of endometriosis.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Endometriosis
/
Neoplasias
Tipo de estudio:
Prognostic_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
Mol Med Rep
Año:
2023
Tipo del documento:
Article
País de afiliación:
Japón