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Nerolidol, Bioactive Compound Suppress Growth of HCT-116 Colorectal Cancer Cells Through Cell Cycle Arrest and Induction of Apoptosis.
Zhao, Xiaoqian; Chinnathambi, Arunachalam; Alharbi, Sulaiman Ali; Natarajan, Nandakumar; Raman, Muthusamy.
Afiliación
  • Zhao X; Nuclear Medicine Department, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, 250021, China.
  • Chinnathambi A; Department of Botany and Microbiology, College of Science, King Saud University, PO Box -2455, Riyadh, 11451, Saudi Arabia.
  • Alharbi SA; Department of Botany and Microbiology, College of Science, King Saud University, PO Box -2455, Riyadh, 11451, Saudi Arabia.
  • Natarajan N; Department of Cellular and Molecular Biology, The University of Texas at Tyler Health Science Center, Tyler, TX, 75708, USA.
  • Raman M; Department of Microbiology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, 600077, Tamil Nadu, India. raman.tanuvas@gmail.com.
Appl Biochem Biotechnol ; 196(3): 1365-1375, 2024 Mar.
Article en En | MEDLINE | ID: mdl-37395945
Colon cancer is the most prevalent cancer and causes the highest cancer-associated mortality in both men and women globally. It has a high incidence and fatality rate, which places a significant burden on the healthcare system. The current work was performed to understand the beneficial roles of nerolidol on the viability and cytotoxic mechanisms in the colon cancer HCT-116 cells. The MTT cytotoxicity assay was done to investigate the effect of nerolidol at different doses (5-100 µM) on the HCT-116 cell viability. The impacts of nerolidol on ROS accumulation and apoptosis were investigated using DCFH-DA, DAPI, and dual staining assays, respectively. The flow cytometry analysis was performed to study the influence of nerolidol on the cell cycle arrest in the HCT-116 cells. The outcomes of the MTT assay demonstrated that nerolidol at different doses (5-100 µM) substantially inhibited the HCT-116 cell viability with an IC50 level of 25 µM. The treatment with nerolidol appreciably boosted the ROS level in the HCT-116 cells. The findings of DAPI and dual staining revealed higher apoptotic incidences in the nerolidol-exposed HCT-116 cells, which supports its ability to stimulate apoptosis. The flow cytometry analysis demonstrated the considerable inhibition in cell cycle at the G0/G1 phase in the nerolidol-exposed HCT-116 cells. Our research showed that nerolidol can inhibit the cell cycle, increase ROS accumulation, and activate apoptosis in HCT-116 cells. In light of this, it may prove to be a potent and salutary candidate to treat colon cancer.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Sesquiterpenos / Apoptosis / Neoplasias del Colon Límite: Female / Humans Idioma: En Revista: Appl Biochem Biotechnol Año: 2024 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Sesquiterpenos / Apoptosis / Neoplasias del Colon Límite: Female / Humans Idioma: En Revista: Appl Biochem Biotechnol Año: 2024 Tipo del documento: Article País de afiliación: China