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Development and validation of a new quantitative reverse transcription PCR assay for the diagnosis of human sporotrichosis.
Marques de Macedo, Priscila; Sturny-Leclère, Aude; Freitas, Dayvison Francis Saraiva; Ghelfenstein-Ferreira, Theo; Gutierrez-Galhardo, Maria Clara; Almeida, Marcos de Abreu; Rodrigues, Anderson Messias; Pautet, Thierry; Hamane, Samia; Almeida-Paes, Rodrigo; Zancopé-Oliveira, Rosely Maria; Alanio, Alexandre.
Afiliación
  • Marques de Macedo P; Laboratory of Clinical Research on Infectious Dermatology, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil.
  • Sturny-Leclère A; Laboratory of Parasitology-Mycology, Groupe Hospitalier Saint-Louis-Lariboisière-Fernand-Widal, Assistance Publique-Hôpitaux de Paris, Paris, France.
  • Freitas DFS; Translational Mycology Group, National Reference Center for Invasive Mycoses and Antifungals, Institut Pasteur, Paris, France.
  • Ghelfenstein-Ferreira T; Laboratory of Clinical Research on Infectious Dermatology, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil.
  • Gutierrez-Galhardo MC; Laboratory of Parasitology-Mycology, Groupe Hospitalier Saint-Louis-Lariboisière-Fernand-Widal, Assistance Publique-Hôpitaux de Paris, Paris, France.
  • Almeida MA; Translational Mycology Group, National Reference Center for Invasive Mycoses and Antifungals, Institut Pasteur, Paris, France.
  • Rodrigues AM; Laboratory of Clinical Research on Infectious Dermatology, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil.
  • Pautet T; Mycology Laboratory, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil.
  • Hamane S; Department of Microbiology, Immunology and Parasitology, Universidade Federal de São Paulo (UNIFESP), São Paulo, Brazil.
  • Almeida-Paes R; Laboratory of Parasitology-Mycology, Groupe Hospitalier Saint-Louis-Lariboisière-Fernand-Widal, Assistance Publique-Hôpitaux de Paris, Paris, France.
  • Zancopé-Oliveira RM; Laboratory of Parasitology-Mycology, Groupe Hospitalier Saint-Louis-Lariboisière-Fernand-Widal, Assistance Publique-Hôpitaux de Paris, Paris, France.
  • Alanio A; Mycology Laboratory, Instituto Nacional de Infectologia Evandro Chagas, Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro, Brazil.
Med Mycol ; 61(7)2023 Jul 06.
Article en En | MEDLINE | ID: mdl-37491705
Sporotrichosis is a deep mycosis with limited laboratorial techniques for fast diagnosis. We developed an assay able to detect the genetic material of fungal agents of sporotrichosis, and validated it in human specimens from patients with this disease, obtaining high positivity and specificity.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Esporotricosis / Sporothrix Tipo de estudio: Diagnostic_studies / Guideline / Screening_studies Límite: Animals / Humans Idioma: En Revista: Med Mycol Asunto de la revista: MEDICINA VETERINARIA / MICROBIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Brasil
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Esporotricosis / Sporothrix Tipo de estudio: Diagnostic_studies / Guideline / Screening_studies Límite: Animals / Humans Idioma: En Revista: Med Mycol Asunto de la revista: MEDICINA VETERINARIA / MICROBIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Brasil