Ebi3 knockout aggravates experimental periodontitis via Th17 polarization.
J Clin Periodontol
; 50(10): 1406-1418, 2023 10.
Article
en En
| MEDLINE
| ID: mdl-37534736
ABSTRACT
AIM:
To investigate the role of Ebi3-related cytokines (i.e., interleukin [IL]-35 and/or IL-27) in experimental periodontitis using Ebi3 knockout (KO) mice. MATERIALS ANDMETHODS:
The maxillary right second molar teeth of Ebi3 KO mice and C57BL/6 mice were tied with a silk ligature to induce periodontitis. Three days after ligation, gingival tissues were collected for gene expression analyses. Five days after ligation, the maxillae were removed for haematoxylin and eosin staining and immunohistochemistry. Seven days after ligation, the maxillae were removed for micro-computed tomography.RESULTS:
The ligated side of Ebi3 KO mice showed intense alveolar bone resorption, which was substantially more pronounced than in wild-type (WT) mice. IL-17A expression was significantly higher in the gingiva of the ligated side of Ebi3 KO mice compared with WT mice. IL-10 expression was significantly lower in Ebi3 KO mice than in WT mice. The ligature-induced alveolar bone resorption in Ebi3 KO mice that received recombinant IL-35 injection was significantly less compared with that in Ebi3 KO mice that received control injection.CONCLUSIONS:
Together, these findings suggest that Th17 cells exacerbate experimental periodontitis in mice lacking Ebi3 and that IL-35 may play a critical role in inhibiting periodontal tissue destruction.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Periodontitis
/
Pérdida de Hueso Alveolar
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Clin Periodontol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Japón