Recent advances in structure-based enzyme engineering for functional reconstruction.
Biotechnol Bioeng
; 120(12): 3427-3445, 2023 12.
Article
en En
| MEDLINE
| ID: mdl-37638646
ABSTRACT
Structural information can help engineer enzymes. Usually, specific amino acids in particular regions are targeted for functional reconstruction to enhance the catalytic performance, including activity, stereoselectivity, and thermostability. Appropriate selection of target sites is the key to structure-based design, which requires elucidation of the structure-function relationships. Here, we summarize the mutations of residues in different specific regions, including active center, access tunnels, and flexible loops, on fine-tuning the catalytic performance of enzymes, and discuss the effects of altering the local structural environment on the functions. In addition, we keep up with the recent progress of structure-based approaches for enzyme engineering, aiming to provide some guidance on how to take advantage of the structural information.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Ingeniería de Proteínas
/
Aminoácidos
Idioma:
En
Revista:
Biotechnol Bioeng
Año:
2023
Tipo del documento:
Article