N-linked glycoproteins and host proteases are involved in swine acute diarrhea syndrome coronavirus entry.
J Virol
; 97(10): e0091623, 2023 10 31.
Article
en En
| MEDLINE
| ID: mdl-37772826
ABSTRACT
IMPORTANCE Gaining insight into the cell-entry mechanisms of swine acute diarrhea syndrome coronavirus (SADS-CoV) is critical for investigating potential cross-species infections. Here, we demonstrated that pretreatment of host cells with tunicamycin decreased SADS-CoV attachment efficiency, indicating that N-linked glycosylation of host cells was involved in SADS-CoV entry. Common N-linked sugars Neu5Gc and Neu5Ac did not interact with the SADS-CoV S1 protein, suggesting that these molecules were not involved in SADS-CoV entry. Additionally, various host proteases participated in SADS-CoV entry into diverse cells with different efficiencies. Our findings suggested that SADS-CoV may exploit multiple pathways to enter cells, providing insights into intervention strategies targeting the cell entry of this virus.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Endopeptidasas
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Porcinos
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Enfermedades de los Porcinos
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Glicoproteínas
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Infecciones por Coronavirus
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Internalización del Virus
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Alphacoronavirus
Límite:
Animals
Idioma:
En
Revista:
J Virol
Año:
2023
Tipo del documento:
Article
País de afiliación:
China