Optimization of high-throughput 16S rRNA gene amplicon sequencing: an assessment of PCR pooling, mastermix use and contamination.

Aggarwal, Dinesh; Rajan, Diana; Bellis, Katherine L; Betteridge, Emma; Brennan, Joe; de Sousa, Catarina; Parkhill, Julian; Peacock, Sharon J; de Goffau, Marcus C; Wagner, Josef; Harrison, Ewan M

Aggarwal, Dinesh; Rajan, Diana; Bellis, Katherine L; Betteridge, Emma; Brennan, Joe; de Sousa, Catarina; Parkhill, Julian; Peacock, Sharon J; de Goffau, Marcus C; Wagner, Josef; Harrison, Ewan M.

Afiliación

Aggarwal D; Department of Medicine, University of Cambridge, Cambridge, UK.
Rajan D; Wellcome Sanger Institute, Hinxton, Cambridge, UK.
Bellis KL; Wellcome Sanger Institute, Hinxton, Cambridge, UK.
Betteridge E; Department of Medicine, University of Cambridge, Cambridge, UK.
Brennan J; Wellcome Sanger Institute, Hinxton, Cambridge, UK.
de Sousa C; Wellcome Sanger Institute, Hinxton, Cambridge, UK.
Carriage Study Team; Department of Medicine, University of Cambridge, Cambridge, UK.
Parkhill J; Wellcome Sanger Institute, Hinxton, Cambridge, UK.
Peacock SJ; Department of Medicine, University of Cambridge, Cambridge, UK.
de Goffau MC; Wellcome Sanger Institute, Hinxton, Cambridge, UK.
Harrison EM; Department of Veterinary Medicine, University of Cambridge, Cambridge, UK.

Microb Genom ; 9(10)2023 Oct.

Article en En | MEDLINE | ID: mdl-37843887

Asunto(s)

Bacterias; Humanos; ARN Ribosómico 16S/genética; Bacterias/genética; Análisis de Secuencia de ADN/métodos; Genes de ARNr; Reacción en Cadena de la Polimerasa/métodos

Palabras clave

16S rRNA gene; PCR drift; PCR pooling; contamination; efficiency; laboratory protocol; mastermix; microbiome; nasal samples

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Bacterias Límite: Humans Idioma: En Revista: Microb Genom Año: 2023 Tipo del documento: Article País de afiliación: Reino Unido

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