Autoimmune anti-D in an RhD-positive young infant: Learning from a rare case.
Vox Sang
; 119(1): 70-73, 2024 Jan.
Article
en En
| MEDLINE
| ID: mdl-37930933
ABSTRACT
BACKGROUND AND OBJECTIVES:
Anti-D is usually immune in nature and is formed in individuals lacking D antigen or having variants/altered D phenotypes. In the Indian population, 93.8% are RhD positive, and R1 R1 is the commonest Rh phenotype. Here we report a rare and interesting case of autoimmune anti-D in an RhD-positive 3-month-old infant leading to warm autoimmune haemolytic anaemia. STUDY DESIGN ANDMETHODS:
Auto-anti-D was detected serologically by immunohaematological techniques such as direct antiglobulin test, antibody detection and identification, dithiothreitol, enzyme treatment, antibody titration and elution. Molecular studies were performed to rule out genetic variants of RhD.RESULTS:
Anti-D was confirmed in eluate and blood group post elution was B RhD positive. On genotyping using the Indian-specific RHD genotyping assay, the sample was found to be negative for the RHD*01W.150 (most common RhD variant in Indians) but positive for RHD exon 5 and RHD exon 10 along with glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The sample was further sequenced for RHD exons 1-10 by Sanger sequencing and found to be a wild type, thus, ruling out the presence of an RhD variant.CONCLUSION:
This case is of interest because of the rare occurrence of autoimmune anti-D in an RhD-positive patient of such a young age (3 months). To the best of our knowledge, only two case reports have been published on autoimmune anti-D in infancy (in 1961 and 1964).Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Sistema del Grupo Sanguíneo Rh-Hr
/
Antígenos de Grupos Sanguíneos
Límite:
Humans
/
Infant
Idioma:
En
Revista:
Vox Sang
Año:
2024
Tipo del documento:
Article
País de afiliación:
India