Evaluation of the impact of polypeptide-p on diabetic rats upon its cloning, expression, and secretion in Saccharomyces boulardii.
Arch Microbiol
; 206(1): 37, 2023 Dec 23.
Article
en En
| MEDLINE
| ID: mdl-38142245
ABSTRACT
This study was designed to evaluate the effectiveness of recombinant polypeptide-p derived from Momordica charantia on diabetic rats. In this research, the optimized sequence of polypeptide-p gene fused to a secretion signal tag was cloned into the expression vector and transformed into probiotic Saccharomyces boulardii. The production of recombinant secretion protein was verified by western blotting, HPLC, and mass spectrometry. To assay recombinant yeast bioactivity in the gut, diabetic rats were orally fed wild-type and recombinant S. boulardii, in short SB and rSB, respectively, at two low and high doses as well as glibenclamide as a reference drug. In untreated diabetic and treated diabetic + SB rats (low and high doses), the blood glucose increased from 461, 481, and 455 (mg/dl), respectively, to higher than 600 mg/dl on the 21st day. Whereas glibenclamide and rSB treatments showed a significant reduction in the blood glucose level. The result of this study promised a safe plant-source supplement for diabetes through probiotic orchestration.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Probióticos
/
Diabetes Mellitus Experimental
/
Saccharomyces boulardii
Límite:
Animals
Idioma:
En
Revista:
Arch Microbiol
Año:
2023
Tipo del documento:
Article
País de afiliación:
Irán