Spider silk enhanced tissue engineering of cartilage tissue: Approach of a novel bioreactor model using adipose derived stromal cells.
J Appl Biomater Funct Mater
; 22: 22808000241226656, 2024.
Article
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| MEDLINE
| ID: mdl-38253568
ABSTRACT
Human cartilage tissue remains a challenge for the development of therapeutic options due to its poor vascularization and reduced regenerative capacities. There are a variety of research approaches dealing with cartilage tissue engineering. In addition to different biomaterials, numerous cell populations have been investigated in bioreactor-supported experimental setups to improve cartilage tissue engineering. The concept of the present study was to investigate spider silk cocoons as scaffold seeded with adipose-derived stromal cells (ASC) in a custom-made bioreactor model using cyclic axial compression to engineer cartilage-like tissue. For chemical induction of differentiation, BMP-7 and TGF-ß2 were added and changes in cell morphology and de-novo tissue formation were investigated using histological staining to verify chondrogenic differentiation. By seeding spider silk cocoons with ASC, a high colonization density and cell proliferation could be achieved. Mechanical induction of differentiation using a newly established bioreactor model led to a more roundish cell phenotype and new extracellular matrix formation, indicating a chondrogenic differentiation. The addition of BMP-7 and TGF-ß2 enhanced the expression of cartilage specific markers in immunohistochemical staining. Overall, the present study can be seen as pilot study and valuable complementation to the published literature.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Factor de Crecimiento Transformador beta2
/
Proteína Morfogenética Ósea 7
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Appl Biomater Funct Mater
Año:
2024
Tipo del documento:
Article
País de afiliación:
Alemania