Nod Factor Lipopolysaccharide Purification to Study Nitrogen-Fixing Bacteria Symbiosis with Legumes.
Methods Mol Biol
; 2751: 237-245, 2024.
Article
en En
| MEDLINE
| ID: mdl-38265721
ABSTRACT
Nod factors (NF) are lipochitooligosaccharides produced by nitrogen-fixing rhizobia bacteria. They are key components of the rhizobia-plant signaling exchange required for symbiosis. Thus, techniques to extract, detect, characterize, and purify NF are crucial for the identification of both rhizobial and plant mechanisms underlying nitrogen-fixing symbiosis. Here, we describe a method for NF detection using radiolabeling and thin-layer chromatography. Furthermore, we describe a technique for purifying NF for downstream analyses.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Rhizobium
/
Bacterias Fijadoras de Nitrógeno
/
Fabaceae
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2024
Tipo del documento:
Article
País de afiliación:
España