Selective adsorption and identification of penicillin G sodium in milk by molecularly imprinted polymer doped carbon dot.
Mikrochim Acta
; 191(4): 186, 2024 03 07.
Article
en En
| MEDLINE
| ID: mdl-38451316
ABSTRACT
A carbon dot (CD) was prepared by o-phenylenediamine and water, which showed bright yellow fluorescence under ultraviolet light irradiation (λ = 580 nm), and verified good fluorescence quenching effect on penicillin G sodium (Png-Na). Using methacrylic acid as a functional monomer, ethylene glycol dimethacrylate as a crosslinker, and Png-Na as a template, a kind of composite microsphere combining CD and molecularly imprinted polymer (MIP) was synthesized by surface-initiated atomic transfer radical polymerization (SI-ATRP). For reasons of comparison, we also prepared MIP without CD and non-imprinted polymers (NIPs). Through static and dynamic adsorption experiments, the maximum adsorption capacity was 47.05 mg g-1 and the equilibrium time was 30 min. High-performance liquid chromatography (HPLC) was utilized to determine the content of Png-Na in the spiked milk samples. A sensitive, rapid, and simple method for determination of Png-Na in food samples was developed. The utilized approach enabled the quantification of Png-Na within the concentration range 20-1000 µg L-1 (with a limit of detection of 5 µg L-1). The recoveries achieved were in the range 93.3-98.2%, with a relative standard deviation of 1.2-4.2%. The results demonstrated that CD@MIP possessed the capability of specific adsorption and fluorescence detection of Png-Na, enabling simultaneous detection and enrichment of Png-Na in real samples.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Leche
/
Polímeros Impresos Molecularmente
Límite:
Animals
Idioma:
En
Revista:
Mikrochim Acta
Año:
2024
Tipo del documento:
Article
País de afiliación:
China