Calcitonin receptor, calcitonin gene-related peptide and amylin distribution in C1/2 dorsal root ganglia.
J Headache Pain
; 25(1): 36, 2024 Mar 14.
Article
en En
| MEDLINE
| ID: mdl-38481170
ABSTRACT
BACKGROUND:
The upper cervical dorsal root ganglia (DRG) are important for the transmission of sensory information associated with the back of the head and neck, contributing to head pain. Calcitonin receptor (CTR)-based receptors, such as the amylin 1 (AMY1) receptor, and ligands, calcitonin gene-related peptide (CGRP) and amylin, have been linked to migraine and pain. However, the contribution of this system to nociception involving the cervical DRG is unclear. Therefore, this study aimed to determine the relative distribution of the CTR, CGRP, and amylin in upper cervical DRG.METHODS:
CTR, CGRP, and amylin immunofluorescence was examined relative to neural markers in C1/2 DRG from male and female mice, rats, and human cases. Immunofluorescence was supported by RNA-fluorescence in situ hybridization examining amylin mRNA distribution in rat DRG.RESULTS:
Amylin immunofluorescence was observed in neuronal soma and fibres. Amylin mRNA (Iapp) was also detected. Amylin and CGRP co-expression was observed in 19% (mouse), 17% (rat), and 36% (human) of DRG neurons in distinct vesicle-like neuronal puncta from one another. CTR immunoreactivity was present in DRG neurons, and both peptides produced receptor signalling in primary DRG cell cultures. CTR-positive neurons frequently co-expressed amylin and/or CGRP (66% rat; 84% human), with some sex differences.CONCLUSIONS:
Amylin and CGRP could both be local peptide agonists for CTR-based receptors in upper cervical DRG, potentially acting through autocrine and/or paracrine signalling mechanisms to modulate neuron function. Amylin and its receptors could represent novel pain targets.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Péptido Relacionado con Gen de Calcitonina
/
Receptores de Calcitonina
Límite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
J Headache Pain
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J. headache pain
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Journal of headache and pain
Asunto de la revista:
MEDICINA INTERNA
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NEUROLOGIA
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PSICOFISIOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Nueva Zelanda