A multiplex real-time RT-qPCR assay for simultaneous detection of porcine epidemic diarrhea virus, porcine deltacoronavirus, and swine acute diarrhea syndrome coronavirus.
Arch Virol
; 169(4): 82, 2024 Mar 23.
Article
en En
| MEDLINE
| ID: mdl-38520595
ABSTRACT
Porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) cause intestinal diseases with similar manifestations in suckling piglets. In this study, we developed a multiplex real-time PCR for differential diagnosis of PEDV, PDCoV, and SADS-CoV. The assay demonstrated high specificity with a detection limit of 5 copies/µl for each virus. The assay specifically detected PEDV, PDCoV, and SADS-CoV and excluded all other swine pathogens circulating in pigs. Furthermore, the assay exhibited satisfactory performance in analyzing clinical samples. The data indicate that the newly developed multiplex real-time PCR method can be applied for differential diagnosis of porcine enteric coronaviruses.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades de los Porcinos
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Infecciones por Coronavirus
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Virus de la Diarrea Epidémica Porcina
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Alphacoronavirus
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Deltacoronavirus
Límite:
Animals
Idioma:
En
Revista:
Arch Virol
Año:
2024
Tipo del documento:
Article