Determination of aztreonam/ceftazidime-avibactam synergism and proposal of a new methodology for the evaluation of susceptibility in vitro.
Diagn Microbiol Infect Dis
; 109(2): 116236, 2024 Jun.
Article
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| MEDLINE
| ID: mdl-38537506
ABSTRACT
We proposed a new methodology, the microelution ATM/CZA (mATM/CZA), based on the antibiotic disc elution and the use of resazurin, for rapid (<4h) determination of in vitro susceptibility to aztreonam combined with ceftazidime-avibactam among Enterobacterales. The mATM/CZA presented excellent accuracy with 1.9 %, 98.1 % and 100 % of major error, specificity and sensitivity, respectively. Furthermore, we assessed synergism between aztreonam and ceftazidime-avibactam in Enterobacterales and Pseudomonas aeruginosa, which was observed in 37/55 Enterobacterales and 31/56 P. aeruginosa. As reference methodologies (checkerboard, time-kill curve) are not compatible with the routine of the clinical microbiology laboratories, mATM/CZA is an important alternative to evaluate susceptibility of the combination in a scenario where its clinical use is increasingly important.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Aztreonam
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Pruebas de Sensibilidad Microbiana
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Ceftazidima
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Combinación de Medicamentos
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Sinergismo Farmacológico
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Compuestos de Azabiciclo
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Antibacterianos
Límite:
Humans
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Año:
2024
Tipo del documento:
Article