Transcriptional signature of durable effector T cells elicited by a replication defective HCMV vaccine.
NPJ Vaccines
; 9(1): 70, 2024 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-38561339
ABSTRACT
Human cytomegalovirus (HCMV) is a leading infectious cause of birth defects and the most common opportunistic infection that causes life-threatening diseases post-transplantation; however, an effective vaccine remains elusive. V160 is a live-attenuated replication defective HCMV vaccine that showed a 42.4% efficacy against primary HCMV infection among seronegative women in a phase 2b clinical trial. Here, we integrated the multicolor flow cytometry, longitudinal T cell receptor (TCR) sequencing, and single-cell RNA/TCR sequencing approaches to characterize the magnitude, phenotype, and functional quality of human T cell responses to V160. We demonstrated that V160 de novo induces IE-1 and pp65 specific durable polyfunctional effector CD8 T cells that are comparable to those induced by natural HCMV infection. We identified a variety of V160-responsive T cell clones which exhibit distinctive "transient" and "durable" expansion kinetics, and revealed a transcriptional signature that marks durable CD8 T cells post-vaccination. Our study enhances the understanding of human T-cell immune responses to V160 vaccination.
Texto completo:
1
Banco de datos:
MEDLINE
Idioma:
En
Revista:
NPJ Vaccines
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos