Activating the healing process: three-dimensional culture of stem cells in Matrigel for tissue repair.
BMC Biotechnol
; 24(1): 36, 2024 May 25.
Article
en En
| MEDLINE
| ID: mdl-38796454
ABSTRACT
BACKGROUND:
To establish a strategy for stem cell-related tissue regeneration therapy, human gingival mesenchymal stem cells (hGMSCs) were loaded with three-dimensional (3D) bioengineered Matrigel matrix scaffolds in high-cell density microtissues to promote local tissue restoration.METHODS:
The biological performance and stemness of hGMSCs under 3D culture conditions were investigated by viability and multidirectional differentiation analyses. A SpragueâDawley (SD) rat full-thickness buccal mucosa wound model was established, and hGMSCs/Matrigel were injected into the submucosa of the wound. Autologous stem cell proliferation and wound repair in local tissue were assessed by histomorphometry and immunohistochemical staining.RESULTS:
Three-dimensional suspension culture can provide a more natural environment for extensions and contacts between hGMSCs, and the viability and adipogenic differentiation capacity of hGMSCs were significantly enhanced. An animal study showed that hGMSCs/Matrigel significantly accelerated soft tissue repair by promoting autologous stem cell proliferation and enhancing the generation of collagen fibers in local tissue.CONCLUSION:
Three-dimensional cell culture with hydrogel scaffolds, such as Matrigel, can effectively improve the biological function and maintain the stemness of stem cells. The therapeutic efficacy of hGMSCs/Matrigel was confirmed, as these cells could effectively stimulate soft tissue repair to promote the healing process by activating the host microenvironment and autologous stem cells.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteoglicanos
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Cicatrización de Heridas
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Colágeno
/
Laminina
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Ratas Sprague-Dawley
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Combinación de Medicamentos
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Andamios del Tejido
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Células Madre Mesenquimatosas
Límite:
Animals
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Humans
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Male
Idioma:
En
Revista:
BMC Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China