Transplantation of olfactory mucosa mesenchymal stromal cells repairs spinal cord injury by inducing microglial polarization.
Spinal Cord
; 62(8): 429-439, 2024 Aug.
Article
en En
| MEDLINE
| ID: mdl-38849489
ABSTRACT
STUDY DESIGN:
Animal studiesOBJECTIVES:
To evaluate the therapeutic effect of olfactory mucosa mesenchymal stem cell (OM-MSCs) transplantation in mice with spinal cord injury (SCI) and to explore the mechanism by which OM-MSCs inhibit neuroinflammation and improve SCI.SETTING:
Xiangya Hospital, Central South University; Affiliated Hospital of Guangdong Medical University.METHODS:
Mice (C57BL/6, female, 6-week-old) were randomly divided into sham, SCI, and SCI + OM-MSC groups. The SCI mouse model was generated using Allen's method. OM-MSCs were immediately delivered to the lateral ventricle after SCI using stereotaxic brain injections. One day prior to injury and on days 1, 5, 7, 14, 21, and 28 post-injury, the Basso Mouse Scale and Rivlin inclined plate tests were performed. Inflammation and microglial polarization were evaluated using histological staining, immunofluorescence, and qRT-PCR.RESULTS:
OM-MSCs originating from the neuroectoderm have great potential in the management of SCI owing to their immunomodulatory effects. OM-MSCs administration improved motor function, alleviated inflammation, promoted the transformation of the M1 phenotype of microglia into the M2 phenotype, facilitated axonal regeneration, and relieved spinal cord injury in SCI mice.CONCLUSIONS:
OM-MSCs reduced the level of inflammation in the spinal cord tissue, protected neurons, and repaired spinal cord injury by regulating the M1/M2 polarization of microglia.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Traumatismos de la Médula Espinal
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Mucosa Olfatoria
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Microglía
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Trasplante de Células Madre Mesenquimatosas
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Ratones Endogámicos C57BL
Límite:
Animals
Idioma:
En
Revista:
Spinal Cord
Asunto de la revista:
NEUROLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China