Protocol for Seahorse analysis of ex vivo mouse brown and white adipose tissues.
STAR Protoc
; 5(2): 103042, 2024 Jun 21.
Article
en En
| MEDLINE
| ID: mdl-38850537
ABSTRACT
The mitochondrial stress test is a gold-standard approach for assessing adipose tissue physiological functions and pathological changes. Here, we present a protocol for conducting Seahorse assays using ex vivo mouse brown and white adipose depots. We describe steps for rehydrating the cartridge, preparing freshly harvested fat depots, placing them onto an islet capture plate, and incubating them in a non-CO2 incubator. We then detail procedures for adding mitochondrial stressor solutions and conducting the mitochondrial stress test using the Seahorse XFe24 Analyzer. For complete details on the use and execution of this protocol, please refer to An et al.1.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Tejido Adiposo Pardo
/
Tejido Adiposo Blanco
/
Mitocondrias
Límite:
Animals
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos