Impacts of sample handling and storage conditions on archiving physiologically active soil microbial communities.
FEMS Microbiol Lett
; 3712024 Jan 09.
Article
en En
| MEDLINE
| ID: mdl-38866716
ABSTRACT
Soil microbial communities are fundamental to ecosystem processes and plant growth, yet community composition is seasonally and successionally dynamic, which interferes with long-term iterative experimentation of plant-microbe interactions. We explore how soil sample handling (e.g. filtering) and sample storage conditions impact the ability to revive the original, physiologically active, soil microbial community. We obtained soil from agricultural fields in Montana and Oklahoma, USA and samples were sieved to 2 mm or filtered to 45 µm. Sieved and filtered soil samples were archived at -20°C or -80°C for 50 days and revived for 2 or 7 days. We extracted DNA and the more transient RNA pools from control and treatment samples and characterized microbial communities using 16S amplicon sequencing. Filtration and storage treatments significantly altered soil microbial communities, impacting both species richness and community composition. Storing sieved soil at -20°C did not alter species richness and resulted in the least disruption to the microbial community composition in comparison to nonarchived controls as characterized by RNA pools from soils of both sites. Filtration significantly altered composition but not species richness. Archiving sieved soil at -20°C could allow for long-term and repeated experimentation on preserved physiologically active microbial communities.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Microbiología del Suelo
/
Manejo de Especímenes
/
Bacterias
/
Microbiota
País/Región como asunto:
America do norte
Idioma:
En
Revista:
FEMS Microbiol Lett
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos