Anti-inflammatory germacrane-type sesquiterpene lactones from Vernonia sylvatica.
Chin J Nat Med
; 22(6): 568-576, 2024 Jun.
Article
en En
| MEDLINE
| ID: mdl-38906603
ABSTRACT
Nine new germacranolides, sylvaticalides A-H (1-9), and three known analogues (10-12) were isolated from the aerial part of Vernonia sylvatica. Their structures were established using comprehensive spectroscopic analysis, including high-resolution electrospray ionization mass spectroscopy (HR-ESI-MS) and 1D and 2D nuclear magnetic resonance (NMR) spectra. Their absolute configurations were determined by X-ray diffraction experiments. The anti-inflammatory activities of all isolated compounds were assessed by evaluating their inhibitory effects on the nuclear factor kappa B (NF-κB) pathway, which was activated by lipopolysaccharide (LPS)-stimulated human THP1-Dual cells, and the interferon-stimulated gene (ISG) pathway, activated by STING agonist MSA-2 in the same cell model. Compounds 1, 2 and 6 showed inhibitory effects on the NF-κB and ISG signaling pathways, with IC50 values ranging from 4.12 to 10.57 µmol·L-1.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
FN-kappa B
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Vernonia
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Sesquiterpenos de Germacrano
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Lactonas
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Antiinflamatorios
Límite:
Humans
Idioma:
En
Revista:
Chin J Nat Med
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Chin. J. Nat. Med
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Chinese journal of natural medicines
Año:
2024
Tipo del documento:
Article
País de afiliación:
China