An atlas of transcribed enhancers across helper T cell diversity for decoding human diseases.
Science
; 385(6704): eadd8394, 2024 Jul 05.
Article
en En
| MEDLINE
| ID: mdl-38963856
ABSTRACT
Transcribed enhancer maps can reveal nuclear interactions underpinning each cell type and connect specific cell types to diseases. Using a 5' single-cell RNA sequencing approach, we defined transcription start sites of enhancer RNAs and other classes of coding and noncoding RNAs in human CD4+ T cells, revealing cellular heterogeneity and differentiation trajectories. Integration of these datasets with single-cell chromatin profiles showed that active enhancers with bidirectional RNA transcription are highly cell type-specific and that disease heritability is strongly enriched in these enhancers. The resulting cell type-resolved multimodal atlas of bidirectionally transcribed enhancers, which we linked with promoters using fine-scale chromatin contact maps, enabled us to systematically interpret genetic variants associated with a range of immune-mediated diseases.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Transcripción Genética
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Linfocitos T CD4-Positivos
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Elementos de Facilitación Genéticos
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Predisposición Genética a la Enfermedad
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Sitio de Iniciación de la Transcripción
Límite:
Humans
Idioma:
En
Revista:
Sci. (N.Y., N.Y.)
/
Science
Año:
2024
Tipo del documento:
Article
País de afiliación:
Japón